GNE antibody (N-Term)

Catalog No. ABIN656219

The Rabbit Polyclonal anti-GNE antibody is suitable to detect GNE in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for GNE antibody (N-Term) (ABIN656219)

Target: GNE (Glucosamine (UDP-N-Acetyl)-2-Epimerase/N-Acetylmannosamine Kinase (GNE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB31032

Product Details anti-GNE Antibody

Binding Specificity: AA 169-197, N-Term

Predicted Reactivity: Ha, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This GNE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human GNE.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: GNE Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

Target Details for GNE

Target: GNE (Glucosamine (UDP-N-Acetyl)-2-Epimerase/N-Acetylmannosamine Kinase (GNE))

Alternative Name: GNE

Background: The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq].

Molecular Weight: 79275

Gene ID: 10020

NCBI Accession: NP_001121699, NP_001177312, NP_001177313, NP_001177317, NP_005467

UniProt: Q9Y223