E2F1 antibody (AA 162-476)

Catalog No. ABIN189993

The Mouse Monoclonal anti-E2F1 antibody (Clone 6B5) (ABIN189993) specifically detects E2F1 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for E2F1 antibody (AA 162-476) (ABIN189993)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 6B5

Product Details anti-E2F1 Antibody

Binding Specificity: AA 162-476

Purpose: Anti-E2F-1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes an epitope within aa 223-322 of human E2F-1.

Cross-Reactivity: Human

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL.
Immunoprecipitation: use at 1-5 μg/mL.
Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: Transcription factor E2F1,Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.,Nucleus,E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1

Gene ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy