COPS5 antibody (AA 1-334)

Catalog No. ABIN190283

The Mouse Monoclonal anti-COPS5 antibody has been validated for WB and IP. It is suitable to detect COPS5 in samples from Mouse.

Quick Overview for COPS5 antibody (AA 1-334) (ABIN190283)

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This COPS5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 8H8-5

Product Details anti-COPS5 Antibody

Binding Specificity: AA 1-334

Purpose: Anti-JAB-1 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human and mouse JAB-1.

Cross-Reactivity: Human, Mouse

Immunogen: Mouse JAB-1 gst-fusion protein (aa 1-334) expressed in E. coli.

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting and
Immunoprecipitation: use at 0.5-1 μg/mL.
Positive controls: 293 cells and recombinant fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Target Details for COPS5

Target: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Alternative Name: COPS5

Background: COP9 signalosome complex subunit 5,Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2. {PubMed:11285227, PubMed:11337588, PubMed:12628923, PubMed:12732143, PubMed:19214193, PubMed:20978819, PubMed:22609399, PubMed:9535219}.,JAB-1 is a 38 kD protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the â€signalosome†which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants which plays an important role in light-mediated plant development. Although the specific function of JAB-1 is unclear, it appears to be involved in targeting proteins for degradation by the proteasome.,Cytoplasm, cytosol, Nucleus, Cytoplasm, perinuclear region, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle,SGN5, Signalosome subunit 5, EC 3.4.-.-, Jun activation domain-binding protein 1

UniProt: Q92905

Pathways: Cell Division Cycle