Mre11 antibody (AA 182-582)

Catalog No. ABIN190284

The Mouse Monoclonal anti-Mre11 antibody (Clone 12D7) (ABIN190284) specifically detects Mre11 in WB, ELISA, IP, IHC (p), IF/ICC, PLA and Func. The antibody is reactive with Human samples.

Quick Overview for Mre11 antibody (AA 182-582) (ABIN190284)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Mre11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (fixed cells) (IF/ICC), Proximity Ligation Assay (PLA), Functional Studies (Func)

Clone: 12D7

Product Details anti-Mre11 Antibody

Binding Specificity: AA 182-582

Purpose: Anti-Mre11 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and rat Mre11.

Cross-Reactivity: Human

Immunogen: GST fusion protein corresponding to aa 182-582 of Mre11.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: Use at 0.5-2 μg/mL. A band of ~80 kDa is detected. Detection of Mre11 in 30 μg of whole cell extracts.
Immunofluorescence: use at 1-5 μg/mL. Detection of Mre11 in HeLa cells fixed in 4 % paraformaldehyde for 15 minutes.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C,-80 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C to-70°C. Store as concentrated solution in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Alternative Name: MRE11

Background: Double-strand break repair protein MRE11,Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:29670289, PubMed:30612738, PubMed:9590181, PubMed:9705271}.,Nucleus, Chromosome, telomere, Chromosome,EC 3.1.-.-, Double-strand break repair protein MRE11A, Meiotic recombination 11 homolog 1, MRE11 homolog 1, Meiotic recombination 11 homolog A, MRE11 homolog A

UniProt: P49959

Pathways: DNA Damage Repair