LIG1 antibody (AA 1-919)

Catalog No. ABIN190291

This Mouse Monoclonal antibody specifically detects LIG1 in WB, IP, IF/ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for LIG1 antibody (AA 1-919) (ABIN190291)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 10H5

Product Details anti-LIG1 Antibody

Binding Specificity: AA 1-919

Purpose: Anti-DNA Ligase I Mouse Monoclonal Antibody

Specificity: This antibody recognizes human DNA Ligase I (125 kD), one of the four ligase proteins found in mammalian cells that are required for the joining of DNA strand breaks that occur during DNA replication, repair, and recombination.

Cross-Reactivity: Human

Immunogen: Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 1-5 μg/mL.
Immunoprecipitation: use at 1-5 μg/mL.
Positive controls: MCF-7 cells or recombinant protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA ligase 1

Background: DNA ligase 1,DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,Nucleus.,EC 6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA