K-RAS antibody (AA 150-189)

Catalog No. ABIN6579257

This Rabbit Polyclonal antibody specifically detects K-RAS in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for K-RAS antibody (AA 150-189) (ABIN6579257)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-K-RAS Antibody

Binding Specificity: AA 150-189

Purpose: K-Ras Antibody

Specificity: K-Ras Polyclonal Antibody detects endogenous levels of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human KRAS. AA range:150-189

Isotype: IgG

Application Details

Application Notes: IHC: 100-300.WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Background:

Synonyms: GTPase KRas (K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras) [Cleaved into: GTPase KRas, N-terminally processed]

Background: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008],

Gene Name: KRAS

Protein Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Molecular Weight: 21 kDa

Gene ID: 3845

OMIM: 190070

UniProt: P01116