E2F4 antibody (AA 61-110)

Catalog No. ABIN6579716

The Rabbit Polyclonal anti-E2F4 antibody is suitable to detect E2F4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for E2F4 antibody (AA 61-110) (ABIN6579716)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-E2F4 Antibody

Binding Specificity: AA 61-110

Purpose: E2F-4 Antibody

Specificity: E2F-4 Polyclonal Antibody detects endogenous levels of E2F-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from human E2F4 around the non-acetylation site of Lys96. AA range:61-110

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background:

Synonyms: E2F4, Transcription factor E2F4, E2F-4

Background: The protein encoded byThis gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated ge

Gene Name: E2F4

Protein Name: Transcription factor E2F4

Molecular Weight: 43 kDa

Gene ID: 1874

OMIM: 600659

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size