CD40 Ligand antibody

Catalog No. ABIN6651981

This Hamster Monoclonal antibody specifically detects CD40 Ligand in FACS and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for CD40 Ligand antibody (ABIN6651981)

Target: CD40 Ligand (CD40LG)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MAB0712

Product Details anti-CD40 Ligand Antibody

Specificity: This antibody detects Mouse CD40 Ligand. Other species not tested. Species: Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Mouse Th1 cells.

Isotype: IgG2

Application Details

Application Notes: Flow Cytometry: 1/100-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/300.

Restrictions: For Research Use only

Handling

Buffer: PBS Stabilizers: None

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: cd154,cd40l

Background: CD40 ligand (CD40L), also known as CD154, TNFSF5, TRAP or gp39, is a 260 amino acid type II transmembrane glycoprotein belonging to the TNF family. Murine CD40L consists of a 22 aa cytoplasmic domain, a 24 aa transmembrane domain, and 214 aa extracellular domain bearing a single glycosylation site. CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including NK cells, mast cells, basophils and eosinophils. Murine CD40L shares 78 % amino acid sequence identity with human CD40L. Native bioactive soluble CD40L exists. Soluble human trimeric CD40L secreted by stimulated T cells has been shown to be generated by proteolysis in the microsomes. Both membrane bound and soluble CD40L induce similar effects on B cells.

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints