Cyclin E1 antibody (AA 10-176)

Catalog No. ABIN6654284

This anti-Cyclin E1 antibody is a Mouse Monoclonal antibody detecting Cyclin E1 in IHC (p). Suitable for Human.

Quick Overview for Cyclin E1 antibody (AA 10-176) (ABIN6654284)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CCNE1-2460

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 10-176

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Recombinant human partial protein (within amino acids 10-176) was used as the immunogen for the Cyclin E1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Cyclin E1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Cyclin E1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1 / CCNE1

Background: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases