APEX1 antibody

Catalog No. ABIN6654475

The Mouse Monoclonal anti-APEX1 antibody is suitable to detect APEX1 in samples from Human. It has been validated for WB, IF, IHC (p), ICC and FACS.

Quick Overview for APEX1 antibody (ABIN6654475)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: CPTC-APEX1-2

Product Details anti-APEX1 Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the APE1 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the APE1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,IF/ICC: 1-2 μg/mL,FACS (intracellular): 1-2 μg/10^6 cells in 0.1ml,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the APE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APE1 / APEX1

Background: APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus