Recombinant MITF antibody (full length)

Catalog No. ABIN6654666

This Rabbit Monoclonal antibody specifically detects MITF in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant MITF antibody (full length) (ABIN6654666)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: MPAF3-2R

Product Details anti-MITF Antibody

Binding Specificity: full length

Purpose: Recombinant MITF Antibody (azide and preservative free)

Purification: Protein A affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant MITF antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant MITF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MITF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF (microphthalmia transcription factor) is a transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. [UniProt]

UniProt: O75030

Pathways: Chromatin Binding