Recombinant MTAP antibody (AA 97-196)

Catalog No. ABIN6654902

The Rabbit Monoclonal anti-MTAP antibody (Clone SMTP-1R) (ABIN6654902) specifically detects MTAP in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant MTAP antibody (AA 97-196) (ABIN6654902)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MTAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SMTP-1R

Product Details anti-MTAP Antibody

Binding Specificity: AA 97-196

Purpose: Recombinant MTAP Antibody / S-methyl-5'-thioadenosine phosphorylase

Purification: Protein A affinity chromatography

Immunogen: A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant MTAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: MTAP

Background: Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process