EPH Receptor B2 antibody (pTyr317)

Catalog No. ABIN6655036

Supplier Product No.: 600-401-d36

The Rabbit Polyclonal anti-EPH Receptor B2 antibody has been validated for WB and ELISA. It is suitable to detect EPH Receptor B2 in samples from Chicken.

Quick Overview for EPH Receptor B2 antibody (pTyr317) (ABIN6655036)

Target: EPH Receptor B2 (EPHB2)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EPH Receptor B2 Antibody

Binding Specificity: pTyr317

Supplier Product No.: 600-401-d36

Supplier: Rockland

Purpose: EphrinB phospho Y317 Antibody

Cross-Reactivity (Details): Anti-EphrinB pY317 antibody is directed against rat Ephrin.

Purification: Anti-EphrinB pY317 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-EphrinB pY317 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of Xenopus EphrinB conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-EphrinB pY317 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB protein phosphorylated at Tyr317 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background:

Synonyms: Ephrin type-B receptor 2, EPH-like kinase 5, EK5

Background: EphrinB Antibody detects rat EphrinB. recognizes EphrinB proteins which are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. Anti-EphrinB pY317 Antibody is ideal for investigators involved in Cell Migration, Cell Division, Cell Signaling and Neuroscience.

Gene Name: EPHB2

Gene ID: 396513

NCBI Accession: NP_996834

UniProt: P28693

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins