ATF6 antibody (N-Term)

Catalog No. ABIN6655071

Supplier Product No.: 600-401-gu9s

This Rabbit Polyclonal antibody specifically detects ATF6 in WB, IHC, ELISA, IP, IF, FACS and FM. It exhibits reactivity toward Human.

Quick Overview for ATF6 antibody (N-Term) (ABIN6655071)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-ATF6 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-gu9s

Supplier: Rockland

Purpose: ATF6 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ATF6.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-ATF6 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human ATF6 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5.0 μg/mL

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-ATF6 Antibody has been tested in Western Blot, IF and ELISA. Recombinant ATF6 protein positive control was used for western blotting. Expect a band at 74.5kDa in western blot using appropriate lysates or recombinant protein. Antibody tested positive in U251 cells (W09-001-GY4) in Immunofluorescence. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6

Background:

Synonyms: rabbit anti-ATF6 Antibody, Activating Transcription Factor 6, rabbit anti-ATF-6 Antibody, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6 alpha

Background: ATF6 belongs to the bZIP (basic-leucine zipper domain transcription factor) family. Activating target genes for the unfolded protein response during endoplasmic reticulum stress. Embeded in the endoplasmic reticulum, it functions as an ER stress sensor/stress transducer. Following stress-induced proteolysis it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in the activation of transcription by the serum response factor. This protein may play a role in the development of the foveal and cone function in the retina. Therefore, attributing to disorders such as achromatopsia, blindness, nystagmus, myopia, cataracts and more. Anti-ATF6 Antibody is useful for researchers studying eye development, protein folding and processing, Endoplasmic Reticulum Stress Pathways, Glucose and Energy Metabolism Research.

Gene Name: ATF6

Gene ID: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response