CD80 antibody (FITC)

Catalog No. ABIN6655081

Supplier Product No.: 200-302-n74

The Mouse Monoclonal anti-CD80 antibody has been validated for FACS, WB and IHC. It is suitable to detect CD80 in samples from Human.

Quick Overview for CD80 antibody (FITC) (ABIN6655081)

Target: CD80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 2D10

Product Details anti-CD80 Antibody (FITC)

Supplier Product No.: 200-302-n74

Supplier: Rockland

Purpose: CD80 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD80.

Purification: Fluorescein conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD80.

Sterility: Sterile filtered

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD80 is tested for Flow Cytometry and is useful for Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7, CD80, CD28LG, CD28LG1, LAB7

Background: CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Gene Name: CD80

Gene ID: 941

NCBI Accession: NP_005182

UniProt: P33681

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints