CD25 antibody (PE)

Catalog No. ABIN6655113

Supplier Product No.: 200-308-n25

This Mouse Monoclonal antibody specifically detects CD25 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD25 antibody (PE) (ABIN6655113)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: BC96

Product Details anti-CD25 Antibody (PE)

Supplier Product No.: 200-308-n25

Supplier: Rockland

Purpose: CD25 Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Purification: Phycoerythrin conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25.

Sterility: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5μL/test/1x10e5 to 1x10e8 cells

Comment:

Anti-CD25 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

Background: The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells. The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on induced peripheral Tregs.

Gene Name: IL2RA

Gene ID: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation