APP antibody

Catalog No. ABIN6655141

Supplier Product No.: 200-401-e87

The Rabbit Polyclonal anti-APP antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect APP in samples from Human. There are 3+ publications available.

Quick Overview for APP antibody (ABIN6655141)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-APP Antibody

Supplier Product No.: 200-401-e87

Supplier: Rockland

Purpose: Amyloid Fibrils (OC) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Amyloid Fibrils (OC) from Human based on 100 % homology with the immunizing sequence.

Purification: Anti-Amyloid Fibrils (OC) Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Amyloid Fibrils (OC) Antibody was produced from whole rabbit serum prepared by repeated immunizations with fibrils prepared from human Abeta42 synthetic peptide.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: Other
Anti-Amyloid Fibrils (OC) Antibody is tested for use in IP, IF microscopy, IHC, and WB. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-APP antibody (ABIN6655141)

Lee, Park, Kim, Lee, Lee, Hwang, Lee, Lee, Yoon: "Plasmonic nanoparticle amyloid corona for screening Aβ oligomeric aggregate-degrading drugs." in: Nature communications, Vol. 12, Issue 1, pp. 639, (2021) (PubMed).

Nnah, Lee, Wessling-Resnick: "Iron potentiates microglial interleukin-1β secretion induced by amyloid-β." in: Journal of neurochemistry, Vol. 154, Issue 2, pp. 177-189, (2020) (PubMed).

Jeong, Kim, Chae, Lee, Yang, Kim, Kim, Lee, Lee, Choi, Yoon, Hwang: "Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40." in: Sensors (Basel, Switzerland), Vol. 18, Issue 6, (2018) (PubMed).

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background:

Synonyms: Amyloid OC, Fibrils, Amyloid Oligomer αβ, A11, Amyloid beta A4 protein, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, APP, A4, AD1

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease.

Gene Name: APP

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour