PML antibody

Catalog No. ABIN6655213

Supplier Product No.: 600-401-x44

The Rabbit Polyclonal anti-PML antibody has been validated for WB and ELISA. It is suitable to detect PML in samples from Human.

Quick Overview for PML antibody (ABIN6655213)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PML Antibody

Supplier Product No.: 600-401-x44

Supplier: Rockland

Purpose: PML Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-PML Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-PML Antibody was produced in rabbits by repeated immunizations with three different synthetic peptides from human PML.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:100 - 1:200

Western_Blot_Dilution: 1:750

Comment:

Anti-PML Antibody is tested for ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 115 and 60 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background:

Synonyms: Protein PML Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19, MYL, PP8675, RNF71, TRIM19

Background: PML is a transcription factor and tumor supressor which belongs to the tripartite motif (TRIM) family. This motif contains three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML regulates the response of p53 to oncogenic signals. It is often involved in a chromosomal translocation between chromosomes 15 and 17 which results in a PML/RARalpha fusion protein and is associated with most cases of acute promyelocytic leukemia. Anti-PML Antibody is ideal for research in Gene Expression, Cell Cycle Control and Cancer.

Gene Name: PML

Gene ID: 5371

NCBI Accession: NP_002666

UniProt: P29590

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus