SMAD6 antibody

Catalog No. ABIN6655220

Supplier Product No.: 200-401-i15

This anti-SMAD6 antibody is a Rabbit Polyclonal antibody detecting SMAD6 in WB, IHC and IF. Suitable for Human.

Quick Overview for SMAD6 antibody (ABIN6655220)

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SMAD6 Antibody

Supplier Product No.: 200-401-i15

Supplier: Rockland

Purpose: SMAD6 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-SMAD6 from Human, Mouse, New World monkey, Rat and Sheep based on 100 % homology with the immunizing sequence.

Purification: Anti-SMAD6 Antibody was purified by Protein G chromatography.

Immunogen: SMAD6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to two internal amino acid sequences in the human SMAD6 protein.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-SMAD6 antibody is tested for use in IHC, IF, and WB. Expect a band approximately 53kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6

Background:

Synonyms: MADH6

Background: Anti-SMAD6 antibody detects human SMAD6. SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Anti-SMAD6 antibody is ideal for investigators involved kinase and phosphatase and NFkappaB research.

Gene Name: SMAD6

Gene ID: 4091

NCBI Accession: NP_001136333

UniProt: O43541

Pathways: Chromatin Binding