IFNAR1 antibody (pSer535, pSer539)

Catalog No. ABIN6655258

Supplier Product No.: 600-401-d67

The Rabbit Polyclonal anti-IFNAR1 antibody has been validated for WB and IHC. It is suitable to detect IFNAR1 in samples from Human.

Quick Overview for IFNAR1 antibody (pSer535, pSer539) (ABIN6655258)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IFNAR1 Antibody

Binding Specificity: pSer535, pSer539

Supplier Product No.: 600-401-d67

Supplier: Rockland

Purpose: Interferon alpha Receptor Type I phospho S535/phospho S539 Antibody

Cross-Reactivity (Details): Anti-Interferon alpha Receptor Type I pS535/pS539 Antibody is directed against human Interferon alpha Receptor Type I phosphorylated at positions S535/S539.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Interferon alpha Receptor Type I pS535/pS539 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Other: Dot Blot: 1:1000

Comment:

Anti-Interferon alpha Receptor Type I pS535/pS539 (Rabbit) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. The molecular weight of the IFNAR1 varies with cell line due to different levels of glycosylation in 293 and HeLa Cells, the mature form is approximately 110-130 kDa in size.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Alternative Name: Interferon alpha Receptor Type I

Background:

Synonyms: Interferon alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1

Background: Interferon alpha Receptor Type I pS535/pS539 Antibody detects Interferon alpha Receptor Type I phopshorylated at positions S535/S539. Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1. Therefore, Interferon alpha Receptor Type I phospho S535/phospho S539 antibody is ideal for investigators involved in Cell Signaling, Virology, Immunology and Cancer Research.

Gene Name: IFNAR1

Gene ID: 3454

UniProt: P17181

Pathways: JAK-STAT Signaling, Hepatitis C