CDC25C antibody

Catalog No. ABIN6655330

Supplier Product No.: 200-301-p28

The Mouse Monoclonal anti-CDC25C antibody has been validated for WB and ELISA. It is suitable to detect CDC25C in samples from Human.

Quick Overview for CDC25C antibody (ABIN6655330)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: DCS-193

Product Details anti-CDC25C Antibody

Supplier Product No.: 200-301-p28

Supplier: Rockland

Purpose: CDC25C Antibody

Cross-Reactivity (Details): This product reacts with human.

Purification: Anti-CDC25C antibody was purified from mouse ascites by protein A chromatography.

Immunogen: CDC25C antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to human Cdc25c.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-CDC25C is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~53.4 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background:

Synonyms: CDC25, PPP1R60, MITOSIS INDUCER CDC25, cell division cycle 25C, protein phosphatase 1, regulatory subunit 60, Dual specificity phosphatase Cdc25C

Background: Cell division sycle(CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34(cdc2)/cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37 % sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31. This antibody is suitable for researchers interested in cancer research, autoimmune disease, cardiovascular disease, neurodegenerative disease, MAK Kinase Signaling, AKT Signaling, GPCR Signaling, Cell Cycle research, and nuclear receptors and transcription factors.

Gene Name: CDC25C

Gene ID: 995

NCBI Accession: NP_001274511

UniProt: P30307

Pathways: Cell Division Cycle, M Phase