MEK1 antibody (pThr292)

Catalog No. ABIN6655358

Supplier Product No.: 600-401-d72

The Rabbit Polyclonal anti-MEK1 antibody (ABIN6655358) specifically detects MEK1 in WB. The antibody is reactive with Human samples.

Quick Overview for MEK1 antibody (pThr292) (ABIN6655358)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MEK1 Antibody

Binding Specificity: pThr292

Supplier Product No.: 600-401-d72

Supplier: Rockland

Purpose: MEK1 phospho T292 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: MEK1 pT292 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-MEK1 pT292 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-MEK1 pT292 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to MEK 1 protein phosphorylated at Thr292 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Background:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1,MAPK/ERK kinase 1

Background: MEK1 pT292 antibody detects MEK1 phosphorylated at Thr292. MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386. Therefore, MEK1 pT292 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Gene Name: MAP2K1

Gene ID: 5604

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling