CD4 antibody (FITC)

Catalog No. ABIN6655531

Supplier Product No.: 200-302-m63

The Mouse Monoclonal anti-CD4 antibody has been validated for FACS and FM. It is suitable to detect CD4 in samples from Human.

Quick Overview for CD4 antibody (FITC) (ABIN6655531)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: RPA-T4

Product Details anti-CD4 Antibody (FITC)

Supplier Product No.: 200-302-m63

Supplier: Rockland

Purpose: CD4 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD4 and chimpanzee.

Purification: Fluorescein conjugated CD4 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against human CD4.

Sterility: Sterile filtered

Immunogen: Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with PHA-stimulated human peripheral blood mononuclear cells (PBMC).

Isotype: IgG1 kappa

Labeling Ratio: 2-8

Application Details

Application Notes:

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD4 has been tested in Flow Cytometry (Cell Surface) and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, FITC, T4

Background: CD4 is an Immunoglobulin Superfamily protein and an accessory protein for MHC class-II antigen/ T-cell receptor interaction. It may regulate T-cell activation. It plays a more general role in mediating cell recognition events than merely those of cellular immune response. The T4 Molecule serves as a receptor for the human immunodeficiency virus. A Type I membrane protein, it is expressed on T lymphocytes, B-cells, macrophages, and granulocytes. It is also expressed in a developmentally regulated manner in specific regions of the brain.

Gene Name: CD4

Gene ID: 920

NCBI Accession: NP_000607

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events