RIPK3 antibody (C-Term)

Catalog No. ABIN6655574

Supplier Product No.: 600-401-i08

This anti-RIPK3 antibody is a Rabbit Polyclonal antibody detecting RIPK3 in WB, IHC, IP and IF. Suitable for Human.

Quick Overview for RIPK3 antibody (C-Term) (ABIN6655574)

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-RIPK3 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-i08

Supplier: Rockland

Purpose: Receptor-interacting serine-threonine kinase 3 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Receptor-interacting serine-threonine kinase 3 from human and chimpanzee based on 100 % homology with the immunizing sequence.

Purification: Anti-Receptor-interacting serine-threonine kinase 3 Antibody was purified by affinity chromatography.

Immunogen: Receptor-interacting serine-threonine kinase 3 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a peptide corresponding to amino acids near the c-terminus of human Receptor-interacting serine/threonine-protein kinase 3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 10 μg/mL

Western_Blot_Dilution: 3-7 μg/mL

Comment:

Anti-Receptor-interacting serine-threonine kinase 3 antibody is tested for use in WB, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 56 kDa (isoform 1) and 27 kDa (isoform 2) on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RIPK3

Target: RIPK3 (Receptor-Interacting serine-threonine Kinase 3 (RIPK3))

Alternative Name: Receptor-interacting serine-threonine kinase 3

Background:

Synonyms: RIP3, Receptor-interacting serine/threonine-protein kinase 3, 2.7.11.1, RIP-like protein kinase 3, Receptor-interacting protein 3

Background: Anti-Receptor-interacting serine-threonine kinase 3 antibody detects human RIP3. RIP3 is a novel member of RIP Ser/Thr kinase family. It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex. RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction. RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling. It is recruited to the TNFR1 signaling complex in a RIP-dependant manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation. RIP3 negatively regulates the TLR3-Trif mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1. The human RIP3 gene is localized in the chromosomal region 14q11.2. Anti-Receptor-interacting serine-threonine kinase 3 Antibody is ideal for investigators involved in apoptosis and NFkappaB research.

Gene Name: RIPK3

Gene ID: 11035

NCBI Accession: NP_006862

UniProt: Q9Y572

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades