RUNX1T1 antibody (Internal Region, N-Term)

Catalog No. ABIN6655575

Supplier Product No.: 100-401-v08

Product Details anti-RUNX1T1 Antibody

Target: RUNX1T1 (Runt-Related Transcription Factor 1, Translocated To, 1 (Cyclin D-Related) (RUNX1T1))

Binding Specificity: Internal Region, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1T1 antibody is un-conjugated

Application: ELISA, Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 100-401-v08

Supplier: Rockland

Purpose: ETO Antibody

Cross-Reactivity (Details): This product reacts with human ETO.

Purification: Anti-ETO Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-ETO Antibody was produced in rabbits by repeated immunizations with human ETO using two synthetic peptides containing sequences from the N-terminal and internal region of the protein respectively.

Isotype: IgG1

Application Details

Application Notes:

ChIP_Dilution: 4 μL/ChIP

ELISA_Dilution: 1:100

Comment:

Anti-ETO Antibody has been tested in ChIP and ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RUNX1T1

Target: RUNX1T1 (Runt-Related Transcription Factor 1, Translocated To, 1 (Cyclin D-Related) (RUNX1T1))

Alternative Name: ETO (RUNX1T1 Products)

Background:

Synonyms: Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMTND2

Background: ETO is a transcriptional regulator which belongs to the myeloid translocation gene family. ETO exerts its function by interaction with transcription factors bound to promoters and binding to histone deacetylases. It recruits a range of corepressors to facilitate transcriptional repression. The t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukaemia. This translocation produces a chimeric gene made up of the 5'-region of AML1 and the 3'-region of the ETO gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Anti-ETO Antibody is ideal for research in Gene Expression, Transcription and Cancer.

Gene Name: RUNX1T1

Gene ID: 862

NCBI Accession: NP_001185554

UniProt: Q06455