TBK1 antibody

Catalog No. ABIN6655825

Supplier Product No.: 200-301-i22

The Mouse Monoclonal anti-TBK1 antibody has been validated for WB, IHC, IP and IF. It is suitable to detect TBK1 in samples from Human.

Quick Overview for TBK1 antibody (ABIN6655825)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 108A429

Product Details anti-TBK1 Antibody

Supplier Product No.: 200-301-i22

Supplier: Rockland

Purpose: TBK1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TBK1 from Human, Mouse, cow, dog and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-TBK1 Antibody was purified by Protein G chromatography.

Immunogen: TBK1 Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide corresponding to internal amino acids of the human protein TBK1.

Isotype: IgG1 kappa

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1-2 μg/mL

Comment:

Anti-TBK1 Antibody is tested for use in WB, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 80kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Alternative Name: TBK1

Background:

Synonyms: NAK, Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, Serine/threonine-protein kinase TBK1, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1

Background: Anti-TBK1 NAK Antibody detects human TBK1 NAK. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing it's translocation to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recently, a new IKK family member, TBK1 [(TANK-binding kinase), also known as NAK and T2K] has been identified. TANK (TRAF-associated NF-kB activator) interacts with TBK1 and activates NF-kB in a kinase dependent manner. Anti-TBK1 NAK Antibody is ideal for investigators involved in NFkappaB and apoptosis research.

Gene Name: TBK1

Gene ID: 29110

NCBI Accession: NP_037386

UniProt: Q9UHD2

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome