TLR4 antibody (Internal Region)

Catalog No. ABIN6655892

Supplier Product No.: 600-401-g52

This anti-TLR4 antibody is a Rabbit Polyclonal antibody detecting TLR4 in WB, FACS, IHC, IF and FM. Suitable for Human.

Quick Overview for TLR4 antibody (Internal Region) (ABIN6655892)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-TLR4 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-g52

Supplier: Rockland

Purpose: TLR4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with TRL 4 from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR4 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: TRL4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against synthetic peptide corresponding to an internal region of human TLR4.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:50

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-TLR4 Antibody has been tested in WB, IHC, IF microscopy and Flow Cytometry. Expect a band approximately ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.5 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background:

Synonyms: ARMD10, CD284, TLR 4, TOLL, Toll like receptor 4

Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediatiors of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa.

Gene Name: TRL4

Gene ID: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins