ERK1 antibody

Catalog No. ABIN6656069

Supplier Product No.: 100-401-f25

This anti-ERK1 antibody is a Rabbit Polyclonal antibody detecting ERK1 in WB, IHC, IF and FACS. Suitable for Rat.

Quick Overview for ERK1 antibody (ABIN6656069)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ERK1 Antibody

Supplier Product No.: 100-401-f25

Supplier: Rockland

Purpose: Erk1/2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Erk1/2 from Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-Erk1/2 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Erk 1/2 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the synthetic peptide coupled to KLH.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:50

Flow_Cytometry_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:5000

Comment:

Anti-Erk1/2 Antibody has been tested in WB, IF microscopy and IHC. Expect a band approximately ~44kda and ~42kDa bands corresponding to the molecular weights of Erk1 and Erk2. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: Mapk3

Background:

Synonyms: Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1, Erk1, Prkm3

Background: The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. They are both located in the cytosol and mitochondria. While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions, the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases. ERK1/2 activation is generally thought to confer a survival advantage to cells, however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis.

Gene Name: Mapk3

Gene ID: 50689

NCBI Accession: NP_059043

UniProt: P21708

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins