PAK1 antibody (pThr84)

Catalog No. ABIN6656323

Supplier Product No.: 600-401-x21

The Rabbit Polyclonal anti-PAK1 antibody has been validated for WB and ELISA. It is suitable to detect PAK1 in samples from Human.

Quick Overview for PAK1 antibody (pThr84) (ABIN6656323)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAK1 Antibody

Binding Specificity: pThr84

Supplier Product No.: 600-401-x21

Supplier: Rockland

Purpose: PAK 1 Phospho T84 Antibody

Cross-Reactivity (Details): Anti-Phospho-Thr84 PAK1 Antibody is directed against PAK1 protein phosphorylated at T84.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Thr84 PAK1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr84 of human PAK1 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Thr84 PAK1 Antibody is tested for Western Blots and is specific for the ~68 kDa PAK1 phosphorylated at Thr84. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background:

Synonyms: Serine/Threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, p65-PAK

Background: PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation. Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif. OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin. PAK1 pT84 Antibody is ideal for researchers interested in cell cycle research.

Gene Name: PAK1

Gene ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis