TLR9 antibody

Catalog No. ABIN6656659

Product Details anti-TLR9 Antibody

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-TLR9 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR9 from monkey based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR9 from other sources has not been determined.

Immunogen:

Immunogen: TLR9 Antibody was produced in mice prepared repeated immunizations with amino acids corresponding to an internal sequence of human TLR9 isoform A protein.

Immunogen Type: Peptide

Clone: 26C593-2

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 10-20 μg/mL (frozen)

Application Note: Anti-TLR9 Antibody is suitable for use in CM, Flow (CS), Flow (I), IF/ICC, IHC (frozen), IHC (p) and WB. Expect a band approximately 113 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 0.1-2 μg/10^6 cells

Western Blot Dilution: 1-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9 (TLR9 Products)

Synonyms: CD289 antibody, toll like receptor 9 antibody, toll-like receptor 9 antibody, TLR9 antibody, Tlr9 antibody

Background:

Synonyms: Toll-like receptor 9

Background: Anti-TLR9 CD289 Antibody detects human TLR9 CD289. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, a cDNA coding for TLR9 has been identified and subsequently isolated. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5 % . TLR9 is highly expressed in spleen. Anti-TLR9 CD289 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR9

Gene ID: 54106

NCBI Accession: NP_059138

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades