GFAP antibody (AA 411-422) (PE)

Catalog No. ABIN6656807

Supplier Product No.: 200-308-w55

The PE-conjugated Mouse Monoclonal anti-GFAP antibody (Clone S206A-8) (ABIN6656807) specifically detects GFAP in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for GFAP antibody (AA 411-422) (PE) (ABIN6656807)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S206A-8

Product Details anti-GFAP Antibody (PE)

Binding Specificity: AA 411-422

Supplier Product No.: 200-308-w55

Supplier: Rockland

Purpose: GFAP Antibody Phycoerythrin

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-GFAP Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-GFAP Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-GFAP R-PE Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 12 months

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

Gene Name: GFAP

Gene ID: 2670

UniProt: P14136