TARDBP antibody (Cleavage Site)

Catalog No. ABIN6657361

Supplier Product No.: 600-401-e43

The Rabbit Polyclonal anti-TARDBP antibody has been validated for WB and IHC. It is suitable to detect TARDBP in samples from Human.

Quick Overview for TARDBP antibody (Cleavage Site) (ABIN6657361)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TARDBP Antibody

Binding Specificity: Cleavage Site

Supplier Product No.: 600-401-e43

Supplier: Rockland

Purpose: TDP43 Antibody

Cross-Reactivity (Details): TDP43 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Purification: Anti-TDP43 antibody is directed against human TDP43.

Immunogen: Anti-TDP43 Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues C-terminal to the caspase-cleavage site (between D219 and V220) of the TAR DNA-binding protein 43.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:1000

Comment:

Anti-TDP43 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 43 kDa in size corresponding to the TDP43 protein in Western Blots of rat brain lysate. Molecular weight is dependent upon the TDP43 species present in the sample, full-length or truncated TDP43. Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TDP43

Background:

Synonyms: TAR DNA-binding protein 43

Background: TDP43 antibody detects TDP43 protein (Tar DNA Binding 43, TARDBP). TDP43 was originally identified as a protein that binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. UV cross-linking of HeLa cell extract revealed a 43 kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis. The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms. Anti-TDP43 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: TARDBP

Gene ID: 23435

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion