EEF1A2 antibody (pSer358)

Catalog No. ABIN6657445

Supplier Product No.: 600-401-x05

The Rabbit Polyclonal anti-EEF1A2 antibody has been validated for WB and ELISA. It is suitable to detect EEF1A2 in samples from Human.

Quick Overview for EEF1A2 antibody (pSer358) (ABIN6657445)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF1A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EEF1A2 Antibody

Binding Specificity: pSer358

Supplier Product No.: 600-401-x05

Supplier: Rockland

Purpose: eEF1A2 Phospho S358 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser358 eEF1A2 Antibody has been tested by Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Alternative Name: eEF1A2

Background:

Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1

Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.

Gene Name: EEF1A2

Gene ID: 1917

NCBI Accession: NP_001949

UniProt: Q05639