JUP antibody

Catalog No. ABIN6657477

Supplier Product No.: 200-301-p27

This Mouse Monoclonal antibody specifically detects JUP in WB, IHC and ELISA. It exhibits reactivity toward Chicken.

Quick Overview for JUP antibody (ABIN6657477)

Target: JUP (Junction Plakoglobin (JUP))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 15F11

Product Details anti-JUP Antibody

Supplier Product No.: 200-301-p27

Supplier: Rockland

Purpose: Catenin gamma (Plakoglobin) Antibody

Cross-Reactivity (Details): This product reacts with human and bovine.

Purification: Anti-Catenin gamma (Plakoglobin) antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Catenin gamma (Plakoglobin) antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to chicken plakoglobin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-Catenin gamma is tested for Immunohistochemistry -F, immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~81.8 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: Catenin gamma (Plakoglobin)

Background:

Synonyms: DP3, PDGB, PKGB, CTNNG, DPIII, ARVD12, Catenin gamma, Desmoplakin III, Desmoplakin-3

Background: Junction Plakoglobin (JUP) ,also knows as catenin gamma, is a major cytoplasmic protein that occurs in a soluble and a membrane-associated form and is the only known constituent common to the submembranous plaques of both kinds of adhering junctions, the desmosomes and the intermediate junctions. It is a component of the cadherin-catenin complex, which is predominantly localized where actin filaments anchor in adherens junctions of epithelial cells. The human plakoglobin gene localizes on chromosome 17q21. Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin. This antibody is suitable for researchers interested in cell adhesion and cancer research.

Gene Name: JUP

Gene ID: 3728

NCBI Accession: NP_002221

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location