PPP3R1 antibody

Catalog No. ABIN6657479

Supplier Product No.: 200-301-p23

The Mouse Monoclonal anti-PPP3R1 antibody (Clone CN-A1) (ABIN6657479) specifically detects PPP3R1 in WB, ELISA and IHC. The antibody is reactive with Cow samples.

Quick Overview for PPP3R1 antibody (ABIN6657479)

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This PPP3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: CN-A1

Product Details anti-PPP3R1 Antibody

Supplier Product No.: 200-301-p23

Supplier: Rockland

Purpose: Calcineurin alpha Antibody

Cross-Reactivity (Details): This product reacts with human, rat, and bovine.

Purification: Anti-Calcineurin alpha antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Calcineurin alpha antibody was produced in mice by repeated immunizations with bovine brain calcineurin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:5000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.25-0.5 μg/mL

Comment:

Anti-Calcineurin alpha is tested for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~19.3 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. Aliquot and stored frozen at -20°C for six months. Avoid repeated freeze and thaw cycles.

Expiry Date: 12 months

Target Details for PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1

Background:

Synonyms: CNA2, CNB, CNB1, CALNB1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

Background: Calcineurin, the Ca(2+)/calmodulin-regulated protein phosphatase, first detected in skeletal muscle and brain, has been found in all cells from yeast to mammals. Calcineurin A alpha (PPP3CA),is located on human chromosomes 4, Chromosomal mapping of the human genes for the calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit. Calcineurin regulates bone formation by the osteoblast. This antibody is suitable for researchers interested in apoptosis, transcription factors, nuclear receptors, cancer research, Immune Response, cardiovascular disease, MAK Kinase Signaling, and AKT Signaling.

Gene Name: PPP3R1

Gene ID: 5534

NCBI Accession: NP_000936

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling