Cyclin A antibody

Catalog No. ABIN6657484

Supplier Product No.: 200-301-p30

The Mouse Monoclonal anti-Cyclin A antibody has been validated for WB, IHC and ELISA. It is suitable to detect Cyclin A in samples from Cow.

Quick Overview for Cyclin A antibody (ABIN6657484)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: CY-A1

Product Details anti-Cyclin A Antibody

Supplier Product No.: 200-301-p30

Supplier: Rockland

Purpose: Cyclin A Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, and bovine.

Purification: Anti-Cyclin A antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Cyclin A antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to bovine cyclin A.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5-1 μg/mL

Other: Immunocytochemistry : 1 μg/mL

Comment:

Anti-Cyclin A is tested for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A

Background:

Synonyms: CCN1, CCNA

Background: The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts. This antibody is suitable for researchers interested in cancer research, apoptosis, autoimmune disease, neurodegenerative disease, cardiovascular disease, MAP Kinase Signaling, AKT Signaling, Inflammatory Signaling, and Cell Cycle research.

Gene Name: CCNA2

Gene ID: 281667

NCBI Accession: NP_001068591

UniProt: P30274

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA