RAGE antibody (Internal Region)

Catalog No. ABIN6657517

Supplier Product No.: 600-401-p67

The Rabbit Polyclonal anti-RAGE antibody has been validated for WB and IHC. It is suitable to detect RAGE in samples from Human. There is 1 publication available.

Quick Overview for RAGE antibody (Internal Region) (ABIN6657517)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RAGE Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-p67

Supplier: Rockland

Purpose: AGER/RAGE Antibody

Cross-Reactivity (Details): Anti-RAGE antibody is directed against human RAGE protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: AGER/RAGE affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human RAGE.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Suggested Applications: IF
Anti-RAGE is tested for Immunohistochemistry-P, -F, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42.8 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 250 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RAGE antibody (ABIN6657517)

Bochi, Ferreira, Del Bianco, Pinto, Rodrigues, Trevisani, Furukawa, Bispo, da Silva, Velosa, Nakandakare, Machado, Teodoro, Passarelli, Catanozi: "Aerobic Exercise Training Reduces Atherogenesis Induced by Low-Sodium Diet in LDL Receptor Knockout Mice." in: Antioxidants (Basel, Switzerland), Vol. 11, Issue 10, (2022) (PubMed).

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: AGER/RAGE

Background:

Synonyms: Receptor for advanced glycosylation end products,

Background: The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment. This antibody is suitable for researchers interested in neuroscience research.

Gene Name: AGER

Gene ID: 177

NCBI Accession: NP_001127

UniProt: Q15109

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins