CD34 antibody (C-Term)

Catalog No. ABIN6657527

Supplier Product No.: 600-401-p90

The Rabbit Polyclonal anti-CD34 antibody (ABIN6657527) specifically detects CD34 in FACS, WB and IHC. The antibody is reactive with Human samples.

Quick Overview for CD34 antibody (C-Term) (ABIN6657527)

Target: CD34

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD34 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-p90

Supplier: Rockland

Purpose: CD34 Antibody

Cross-Reactivity (Details): Anti-CD34 antibody is directed against human CD34 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: CD34 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human CD34.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Flow_Cytometry_Dilution: 1-3 μg/1x106 cells

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Comment:

Anti-CD34 is tested for Flow Cytometry, Immunohistochemistry-P, -F, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~40.7 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Synonyms: Hematopoietic progenitor cell antigen CD34, CD34 antibody

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis. This antibody is suitable for researchers interested in stem cell, cancer and neuroscience research.

Gene Name: CD34

Gene ID: 947

NCBI Accession: NP_001020280

UniProt: P28906