Cytokeratin 18 antibody

Catalog No. ABIN6657540

Supplier Product No.: 200-301-p33

This Mouse Monoclonal antibody specifically detects Cytokeratin 18 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 18 antibody (ABIN6657540)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: CY-90

Product Details anti-Cytokeratin 18 Antibody

Supplier Product No.: 200-301-p33

Supplier: Rockland

Purpose: Cytokeratin Peptide 18 Antibody

Cross-Reactivity (Details): This product reacts with human and rat.

Purification: Anti-Cytokeratin Peptide 18 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Cytokeratin Peptide 18 antibody was produced in mice by repeated immunizations with the human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-2 μg/mL

Western_Blot_Dilution: 0.5-1 μg/mL

Comment:

Anti-Cytokeratin Peptide 18 is tested for Immunohistochemistry-F, Immunohistochemistry-P, Immunofluorescence, and Western Blot. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract. western blot tested in human. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin Peptide 18

Background:

Synonyms: K18, Keratin-18, CK-18, Cytokeratin-18, CYK18, Cell proliferation-inducing gene 46 protein

Background: Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.

Gene Name: KRT18

Gene ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis