Hemagglutinin antibody

Catalog No. ABIN6657572

Product Details anti-Hemagglutinin Antibody

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus H5N1

Virus Strain: A/Vietnam/1203/04

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)

Purification: This product was clarified from mouse ascitic fluid and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)].  VN04-16 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza virus representatives of different clades and subclades of the H5 HA subtype.

Immunogen:

Immunogen: This monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 mug HA with incomplete Freund's adjuvant, administered 3 week apart.

Immunogen Type: Native Protein

Clone: 18E1

Isotype: IgG2a kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.

Neutralization Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

0.01 % (w/v) Sodium Azide

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References for anti-Hemagglutinin antibody (ABIN6657572)

Reed, Yen, DuBois, Bridges, Salomon, Webster, Russell: "Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein." in: Journal of virology, Vol. 83, Issue 8, pp. 3568-80, (2009) (PubMed).

Song, Zhang, Yun, Poussard, Smith, Smith, Borisevich, Linde, Zacks, Li, Kavita, Reiserova, Liu, Dumuren, Balasubramanian, Weaver, Parent, Umlauf, Liu, Huleatt, Tussey, Paessler: "Superior efficacy of a recombinant flagellin:H5N1 HA globular head vaccine is determined by the placement of the globular head within flagellin." in: Vaccine, Vol. 27, Issue 42, pp. 5875-84, (2009) (PubMed).

Govorkova, Webby, Humberd, Seiler, Webster: "Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge." in: The Journal of infectious diseases, Vol. 194, Issue 2, pp. 159-67, (2006) (PubMed).

Hanson, Boon, Lim, Webb, Ooi, Webby: "Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice." in: Respiratory research, Vol. 7, pp. 126, (2006) (PubMed).

Hoffmann, Lipatov, Webby, Govorkova, Webster: "Role of specific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 36, pp. 12915-20, (2005) (PubMed).

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Alternative Name: Hemagglutinin (HA Products)

Synonyms: hemagglutinin antibody, Hemagglutinin antibody, HA antibody, HA antibody, ha antibody

Target Type: Influenza Protein

Background:

Synonyms: mouse anti-H5N1 antibody, mouse anti-Hemagglutinin A antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody

Background: Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza.  This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

Gene Name: HA

Gene ID: 159144921

UniProt: A8UDQ2