DNMT3A antibody (C-Term)

Catalog No. ABIN6657590

Supplier Product No.: 200-301-h70

This anti-DNMT3A antibody is a Mouse Monoclonal antibody detecting DNMT3A in WB, IHC, IF, FACS, ChIP, FM and MA. Suitable for Mouse.

Quick Overview for DNMT3A antibody (C-Term) (ABIN6657590)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: 64B1446

Product Details anti-DNMT3A Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-301-h70

Supplier: Rockland

Purpose: Dnmt3a Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Dnmt3a from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Dnmt3a Antibody was purified by Protein G chromatography.

Immunogen: Dnmt3a Antibody was produced in mice prepared by repeated immunizations with bacteria expressed near the C-terminus recombinant mouse Dnmt3a protein.

Isotype: IgG1 kappa

Application Details

Application Notes:

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 2 μg/mL

Comment:

Anti-Dnmt3a Antibody is tested for use in WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, and IHC-P. Expect a band approximately 120 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a

Background:

Synonyms: DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, 2.1.1.37, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA

Background: Anti-Dnmt3a Antibody detects mouse Dnmt3a. Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. Anti-Dnmt3a Antibody is ideal for investigators involved in caner, epigentics, and cell cycle protein research.

Gene Name: Dnmt3a

Gene ID: 13435

NCBI Accession: NP_031898

UniProt: O88508