CD80 antibody (PE)

Catalog No. ABIN6657769

Supplier Product No.: 200-b08-n89

The Hamster Monoclonal anti-CD80 antibody has been validated for FACS, IHC, IP and FM. It is suitable to detect CD80 in samples from Mouse.

Quick Overview for CD80 antibody (PE) (ABIN6657769)

Target: CD80

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: 16-10A1

Product Details anti-CD80 Antibody (PE)

Supplier Product No.: 200-b08-n89

Supplier: Rockland

Purpose: CD80 Phycoerythrin Antibody

Cross-Reactivity (Details): Cross reactivity with CD80 from other sources has not been tested.

Purification: Phycoerythrin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80.

Sterility: Sterile filtered

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.

Isotype: IgG

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD80 is tested for FLOW and useful for Immunohistochemistry, Immunofluorescence, and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background:

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Background: Anti-CD80 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Gene Name: Cd80

Gene ID: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints