CD25 antibody (APC)

Catalog No. ABIN6657775

Supplier Product No.: 200-526-m73

The Rat Monoclonal anti-CD25 antibody has been validated for FACS. It is suitable to detect CD25 in samples from Mouse.

Quick Overview for CD25 antibody (APC) (ABIN6657775)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: PC61

Product Details anti-CD25 Antibody (APC)

Supplier Product No.: 200-526-m73

Supplier: Rockland

Purpose: CD25 APC Antibody

Cross-Reactivity (Details): Reactivity is observed against mouse CD25.

Purification: Allophycocyanin conjugated CD25 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against mouse CD25.

Sterility: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent cytolytic mouse T-cell clone B6.1.

Isotype: IgG1 lambda

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 0.1-0.5 μg/1x10^6 cells

Comment:

Anti-CD25 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25 Antibody

Background: CD25 is a 55KDa transmembrane glycoprotein consisting of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. It is expressed during early stages of thymocytes development and in activated CD4+ memory T-lymphocytes. CD25 heterodimerizes with IL2R-beta and IL-2R gamma and forms the tripartite high affinity IL-2 receptor complex. It increases the specificity and affinity of IL-2 receptor complex for IL-2 ligand and is necessary for receptor clustering required for augmentation of IL-2 signaling. Mutations in CD25 result in severe immunodeficiency.

Gene Name: IL2RA

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation