CD25 antibody (APC)

Catalog No. ABIN6657776

Supplier Product No.: 200-526-n82

The APC-conjugated Rat Monoclonal anti-CD25 antibody (Clone 3C7) (ABIN6657776) specifically detects CD25 in FACS and IP. The antibody is reactive with Mouse samples.

Quick Overview for CD25 antibody (APC) (ABIN6657776)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 3C7

Product Details anti-CD25 Antibody (APC)

Supplier Product No.: 200-526-n82

Supplier: Rockland

Purpose: CD25 Allophycocyanin Antibody

Cross-Reactivity (Details): Cross reactivity with CD25 from other sources has not been tested.

Purification: Allophycocyanin conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD25.

Sterility: Sterile filtered

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with IL-2-dependent BALB/c mouse helper T-cell clone HT-2.

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Comment:

Anti-CD25 is tested for Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background:

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25, Il2ra, Il2r

Background: CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Gene Name: Il2r

Gene ID: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation