STAT4 antibody (AA 730-749)

Catalog No. ABIN6657793

Supplier Product No.: 100-401-861

The Rabbit Polyclonal anti-STAT4 antibody has been validated for WB, ELISA and IP. It is suitable to detect STAT4 in samples from Mouse.

Quick Overview for STAT4 antibody (AA 730-749) (ABIN6657793)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-STAT4 Antibody

Binding Specificity: AA 730-749

Supplier Product No.: 100-401-861

Supplier: Rockland

Purpose: Stat4 Antibody

Cross-Reactivity (Details):  Reactivity occurs against mouse STAT4 protein.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 725-749 of Mouse STAT-4.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: Use 5 μL

ELISA_Dilution: 1:5,000 - 1:25,000

Western_Blot_Dilution: 1:5,000

Other: User Optimized

Comment:

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  This product was assayed by western blotting after immunoprecipitation of mouse STAT4 from a lysate containing over expressed protein. A 1:5,000 dilution shows reactivity with a band at 85 kDa corresponding to mouse STAT4. Use 5 μL for immunoprecipitation. A HeLa whole cell lysate or mouse testis lysate are suitable for use as a positive control. Reactivity in other immunoassays is unknown. 

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: STAT4

Background:

Synonyms: rabbit anti-STAT4 Antibody, Signal transducer and activator of transcription 4, Stat4

Background: Signal transducer and activator of transcription 4 (STAT4) carries out a dual function of signal transduction and activation of transcription.  STAT4 forms a homodimer or a heterodimer with related transcription factor STAT family members.  STAT4 has a cytoplasmic localization and is translocated into the nucleus in response to activation by JAK, which phosphorylates STAT4 at a tyrosine residue. Various ligands, including interferons and growth factors, induce activation of the JAK kinases, which induce STAT activation. IL-12 has also been identified as an activator of STAT4. Serine phosphorylation is also required for maximal transcriptional activity. The highest level of STAT4 expression is observed in testis and in myeloid cells.

Gene Name: STAT4

Gene ID: 20849

UniProt: P42228

Pathways: JAK-STAT Signaling