Ly6g antibody (APC)

Catalog No. ABIN6657868

Product Details anti-Ly6g Antibody (APC)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Ly6g antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity with Ly-6G (Gr-1) from other sources has not been tested

Purification: Allophycocyanin conjugated Ly-6G (Gr-1) Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse Ly-6G (Gr-1). . Anti-Ly-6G (Gr-1) is conjugated with APC under optimal conditions and the solution is free of unconjugated APC. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C.

Immunogen:

Immunogen: Anti-Ly-6G (Gr-1) Antibody (Monoclonal) was produced by repeated immunizations with Ly-6G/Ly-6C (Gr-1) antigen.

Clone: RB6-8C5

Isotype: IgG2b

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Ly-6G (Gr-1) is useful for Immunohistochemistry, Immunoprecipitation, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Target Details for Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Alternative Name: Ly-6G (Gr-1) (Ly6g Products)

Synonyms: Gr-1 antibody, Gr1 antibody, Ly-6G antibody, lymphocyte antigen 6 complex, locus G antibody, Ly6g antibody

Background:

Synonyms: Ly-6G/Ly-6C (Gr-1), RB6-8C5, Gr-1, Ly-6G, Ly-6C

Background: The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.

Gene Name: Gr-1

Gene ID: 17067

NCBI Accession: NP_001238984