CD14 antibody (Internal Region)

Catalog No. ABIN6657883

Supplier Product No.: 600-401-r04

The Rabbit Polyclonal anti-CD14 antibody is suitable to detect CD14 in samples from Mouse. It has been validated for WB.

Quick Overview for CD14 antibody (Internal Region) (ABIN6657883)

Target: CD14

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD14 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-r04

Supplier: Rockland

Purpose: CD14 Antibody

Cross-Reactivity (Details): Anti-CD14 antibody is directed against mouse CD14 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: CD14 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse CD14.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 0.5 μg/mL

Comment:

Anti-CD14 is tested for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~39.2 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Myeloid cell-specific leucine-rich glycoprotein,CD_antigen=CD14,

Background: CD14, Cluster of differentiation 14, single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns. This antibody is suitable for researchers interested in stem cell research.

Gene Name: CD14

Gene ID: 12475

NCBI Accession: NP_033971

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades