CD4 antibody (FITC)

Catalog No. ABIN6657901

Supplier Product No.: 200-502-m70

The Rat Monoclonal anti-CD4 antibody has been validated for FACS and FM. It is suitable to detect CD4 in samples from Mouse.

Quick Overview for CD4 antibody (FITC) (ABIN6657901)

Target: CD4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: GK1-5

Product Details anti-CD4 Antibody (FITC)

Supplier Product No.: 200-502-m70

Supplier: Rockland

Purpose: CD4 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against mouse CD4.

Purification: Fluorescein conjugated CD4 Monoclonal Antibody was Protein G Purified and is directed against mouse CD4.

Sterility: Sterile filtered

Immunogen: Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with Mouse CTL clone V4.

Isotype: IgG2b kappa

Labeling Ratio: 2-8

Application Details

Application Notes:

Flow_Cytometry_Dilution: 0.1-1 μg/10^6 cells

IF_Microscopy_Dilution: User Optimized

Other: Sufficient to run approximately 500 tests

Comment:

Suggested Applications: Sufficient to run approximately 500 tests
Anti-CD4 is tested in Flow Cytometry (Cell Surface) and useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background:

Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4 Antibody, L3T4, T4

Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily. On T cells, it is the co-receptor for the T cell receptor (TCR) and amplifies the signal generated by the TCR by recruiting the tyrosine kinase lck that is essential for activating many molecules involved in the signaling cascade of an activated T cell. It is an important component of the immune system and is also the cellular receptor for HIV-1. It consists of a cytoplasmic tail, one transmembrane region, and four extracellular domains, D1-D4.  It maps to 12pter-p12 region of human chromosome.

Gene Name: CD4

Gene ID: 12504

NCBI Accession: NP_038516

UniProt: P06332

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events