GADD45G antibody (AA 142-159)

Catalog No. ABIN6658028

Supplier Product No.: 100-401-863

This anti-GADD45G antibody is a Rabbit Polyclonal antibody detecting GADD45G in WB, ELISA and IP. Suitable for Mouse.

Quick Overview for GADD45G antibody (AA 142-159) (ABIN6658028)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GADD45G antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-GADD45G Antibody

Binding Specificity: AA 142-159

Supplier Product No.: 100-401-863

Supplier: Rockland

Purpose: GADD45 gamma Antibody

Cross-Reactivity (Details): Reactivity occurs against mouse PI3K p110d subunit.

Purification: This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 142-159 of Mouse GADD45 gamma. Swiss Prot accession number Q9Z111. This sequence represents the C-terminal end of the protein.  Mouse and rat GADD45 g show 100% sequence homology.   A single amino acid change is found in the human sequence (see below).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: Use 10 μL

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1:100 - 1:500

Other: User Optimized

Comment:

This antibody has been tested for use in ELISA, western blotting and immunoprecipitation.  Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing over expressed protein. A 1:100 to 1:500 dilution shows reactivity with a band at 19.2kDa corresponding to mouse GADD45 gamma. Use 10 μL for immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Alternative Name: GADD45 gamma

Background:

Synonyms: rabbit anti-GADD45 gamma Antibody, Cytokine responsive protein CR6 antibody, DDIT2 antibody, GADD45G antibody antibody, Growth arrest and DNA damage inducible gamma antibody, GRP17 antibody

Background: GADD45 gamma (Growth arrest and DNA-damage-inducible protein also known as cytokine responsive protein CR6) is derived from a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45 gamma is highly expressed in placenta.

Gene Name: GADD45 gamma

Gene ID: 23882

NCBI Accession: NP_035947

UniProt: Q9Z111

Pathways: Cell Division Cycle