NEFM antibody (C-Term)

Catalog No. ABIN6658052

Supplier Product No.: 912-301-d84

This anti-NEFM antibody is a Mouse Monoclonal antibody detecting NEFM in WB, IHC and FM. Suitable for Rat.

Quick Overview for NEFM antibody (C-Term) (ABIN6658052)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: 3H11

Product Details anti-NEFM Antibody

Binding Specificity: C-Term

Supplier Product No.: 912-301-d84

Supplier: Rockland

Purpose: Neurofilament M Antibody

Cross-Reactivity (Details): Anti-NF-M antibody is directed against NF-M protein.

Purification: The antibody is protein G purified from concentrated clarified cell culture supernate.

Immunogen: Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NFM antibody is tested for use in Western Blot, IHC, and IF. Expect a band of approximately 145 kDa in size corresponding to the NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament M

Background:

Synonyms: mouse anti-Neurofilament M Antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-NF-M antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

Gene Name: NEFM

Gene ID: 24588

UniProt: P12839

Pathways: Brown Fat Cell Differentiation